PGEX2-T based β defensin primer designing and isolation of neutrophil, macrophage, bone marrow and mesenchymal stem cells

Defensins are antimicrobial peptides that act mainly by disrupting the structure of bacterial cell membranes and found in many compartments body. play a central role defense against pathogens they considered part innate immune response. Material methods-Reagents: Animal culture media - Growth medium D-MEM, RPMI alpha -MEM was purchased from gibco, CA, USA. Fetal bovine serum (FBS)was obtained Invitrogen, DAPI, agarose SRL, India. Penicillium streptomycin solution himedia, Mumbai, modal: In bred populations Swiss albino mice either sex at 6-8 weeks age were used. Mice House, All India Institute Medical Science, NewDelhi-110029 Standardization, Isolation purification characterization bone marrow mice. Bone is consisting multiple type stem cells such as -Mesenchymal (MSCs) which multipotent have potential to self-renew differentiate into variety specialized types osteoblasts, chondrocytes, adipocytes, neurons. Result: Cells harvested adherence purification, symbol macrophage acentric nucleus visible DAPI stain. The extended cytoplasmic surface CD14 positive staining show purified 98% purity. BM-MSC plastic dish Mesenchymal nucleusis Conclusion: This procedure widely used study biology since moderate numbers resident, unstimulated macrophages can easily be fromthe peritonealcavity.